Introduction to Experimental Techniques in Molecular Biology
Week 1: Introduction to Laboratory Safety and Equipment
Lecture: Laboratory safety protocols, emergency procedures, and equipment overview.
Lab: Familiarization with lab equipment (e.g., pipettes, microscopes, centrifuges).
Week 2: Measurement and Data Collection
Lecture: Units of measurement, precision, and accuracy.
Lab: Practice measuring liquids, solids, and using analytical balances.
Week 3: Basic Techniques in [Subject]
Lecture: Overview of fundamental techniques (e.g., titration, PCR, spectroscopy).
Lab: Hands-on practice with selected techniques.
Week 4: Experiment Design and Controls
Lecture: Hypothesis formulation, variables, and controls.
Lab: Design a simple experiment with proper controls.
Week 5-10: Thematic Experiments
Each week focuses on a specific theme or technique:
Week 5: [Technique/Experiment 1] (Example: DNA Extraction and Gel Electrophoresis)
Week 6: [Technique/Experiment 2] (Example: Acid-Base Titration)
Week 7: [Technique/Experiment 3] (Example: Spectrophotometry)
Week 8: [Technique/Experiment 4] (Example: Bacterial Transformation)
Week 9: [Technique/Experiment 5] (Example: Thin-Layer Chromatography)
Week 10: [Technique/Experiment 6] (Example: Enzyme Kinetics)
Week 11: Data Analysis and Interpretation
Lecture: Introduction to statistical tools (e.g., t-tests, ANOVA, regression analysis).
Lab: Analyze data from previous experiments using software (e.g., Excel, R, or Python).
Week 12: Final Project Presentations
Students present their findings from a mini-research project conducted during the course.
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